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mouse anti human ccl20 mab  (R&D Systems)


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    R&D Systems mouse anti human ccl20 mab
    Mouse Anti Human Ccl20 Mab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+ccl20+mab/Human+CCR6+Antibody/pm22048239-71-20-24
    Average 92 stars, based on 30 article reviews
    mouse anti human ccl20 mab - by Bioz Stars, 2026-09
    92/100 stars

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    Fig. 4. TLR5 immunostaining, increased chemokine expression and mucosal neutrophil influx in H7 flagellin stimulated human colon xenografts. A. Sections of unstimulated human colon xenograft were stained with anti-TLR5 (red) (panel A), an isotype control antibody (panel D), or anti- TLR5 antibody preabsorbed with TLR5 peptide (panel G). Panels B, E and H are the same sections stained with Alexa 488-phalloidin for F-actin (green). Panels C, F and I are merged images of panels A and B, D and E and G and H respectively. Original magnification 400 ×. B. EHEC H7 flagellin or PBS was instilled in the lumen of human colon xenografts. Xenografts were harvested 6 h later, epithelial cells were isolated and CXCL8 mRNA expression levels were determined by real-time PCR. Data are mean + SEM from three xenografts. *P < 0.01 compared with PBS control. C. Human intestinal xenografts were injected with EHEC H7 flagellin (panel A) or PBS (panel C), harvested 6 h later and immunostained for <t>CCL20.</t> No immunostaining was seen with an isotype control (not shown). Panels B and D are nuclear counterstaining of panels A and C, respectively, with Hoechst 33258 dye. Original magnification 200 ×. D. Human intestinal xenografts injected with EHEC H7 flagellin (top panel) or PBS (bottom panel), were harvested after 6 h and stained with hematoxylin and eosin. Top panel shows a small cell infiltrate consisting mostly of neutrophils (arrows); original magnification 400 ×.
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    Fig. 4. TLR5 immunostaining, increased chemokine expression and mucosal neutrophil influx in H7 flagellin stimulated human colon xenografts. A. Sections of unstimulated human colon xenograft were stained with anti-TLR5 (red) (panel A), an isotype control antibody (panel D), or anti- TLR5 antibody preabsorbed with TLR5 peptide (panel G). Panels B, E and H are the same sections stained with Alexa 488-phalloidin for F-actin (green). Panels C, F and I are merged images of panels A and B, D and E and G and H respectively. Original magnification 400 ×. B. EHEC H7 flagellin or PBS was instilled in the lumen of human colon xenografts. Xenografts were harvested 6 h later, epithelial cells were isolated and CXCL8 mRNA expression levels were determined by real-time PCR. Data are mean + SEM from three xenografts. *P < 0.01 compared with PBS control. C. Human intestinal xenografts were injected with EHEC H7 flagellin (panel A) or PBS (panel C), harvested 6 h later and immunostained for CCL20. No immunostaining was seen with an isotype control (not shown). Panels B and D are nuclear counterstaining of panels A and C, respectively, with Hoechst 33258 dye. Original magnification 200 ×. D. Human intestinal xenografts injected with EHEC H7 flagellin (top panel) or PBS (bottom panel), were harvested after 6 h and stained with hematoxylin and eosin. Top panel shows a small cell infiltrate consisting mostly of neutrophils (arrows); original magnification 400 ×.

    Journal: Cellular microbiology

    Article Title: Role of Shiga toxin versus H7 flagellin in enterohaemorrhagic Escherichia coli signalling of human colon epithelium in vivo.

    doi: 10.1111/j.1462-5822.2005.00673.x

    Figure Lengend Snippet: Fig. 4. TLR5 immunostaining, increased chemokine expression and mucosal neutrophil influx in H7 flagellin stimulated human colon xenografts. A. Sections of unstimulated human colon xenograft were stained with anti-TLR5 (red) (panel A), an isotype control antibody (panel D), or anti- TLR5 antibody preabsorbed with TLR5 peptide (panel G). Panels B, E and H are the same sections stained with Alexa 488-phalloidin for F-actin (green). Panels C, F and I are merged images of panels A and B, D and E and G and H respectively. Original magnification 400 ×. B. EHEC H7 flagellin or PBS was instilled in the lumen of human colon xenografts. Xenografts were harvested 6 h later, epithelial cells were isolated and CXCL8 mRNA expression levels were determined by real-time PCR. Data are mean + SEM from three xenografts. *P < 0.01 compared with PBS control. C. Human intestinal xenografts were injected with EHEC H7 flagellin (panel A) or PBS (panel C), harvested 6 h later and immunostained for CCL20. No immunostaining was seen with an isotype control (not shown). Panels B and D are nuclear counterstaining of panels A and C, respectively, with Hoechst 33258 dye. Original magnification 200 ×. D. Human intestinal xenografts injected with EHEC H7 flagellin (top panel) or PBS (bottom panel), were harvested after 6 h and stained with hematoxylin and eosin. Top panel shows a small cell infiltrate consisting mostly of neutrophils (arrows); original magnification 400 ×.

    Article Snippet: Mouse antihuman IL-8 (CXCL8) monoclonal antibody (MAb), biotinylated goat anti-human IL-8 (CXCL8) antibody and mouse anti-human MIP3α (CCL20) MAb were from R and D systems (Minneapolis, MN).

    Techniques: Immunostaining, Expressing, Staining, Control, Isolation, Real-time Polymerase Chain Reaction, Injection